Flow Cytometry analysis of U20S cells using anti-IL7R alpha antibody (PB9948).
Overlay histogram showing U20S cells stained with PB9948 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-IL7R alpha antibody (PB9948).
Overlay histogram showing U87 cells stained with PB9948 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.